Short CV/Education and training

  • 1991
    Secondary School, Abitur (A-levels)

  • 1991 – 1996
    Studied biochemistry at the Classical University of Lisbon, Faculty of Sciences, Lisbon, Portugal.

  • 1995 – 1996
    Diplom degree from the New University of Lisbon, Faculty of Sciences and Technology (UNL-FCT), Department of Chemistry, Lisbon.

  • 1996 – 1997
    Research assistant at UNL-FCT, Department of Chemistry, Lisbon.

  • 1998 – 2002
    PhD in biochemistry and biophysics at UNL-FCT, Department of Chemistry, Lisbon.

  • 2002
    Postdoctoral scholar at California Institute of Technology, Department of Chemistry and Chemical Engineering, Pasadena, USA.

  • 2003 – 2005
    Postdoctoral scholar at the University of Göttingen, Department of Molecular Structural Biology, Göttingen, Germany.

  • 2006 – 2012
    Emmy Noether research group director at the University of Freiburg, Department of Organic Chemistry and Biochemistry, Freiburg, Germany.

  • Since 2012
    Professor for Biochemistry (W2) at the University of Freiburg, Department Biochemistry, Freiburg, Germany.

Selected publications

  • Helfmann, S. et al.: Cooperative binding of ATP and ADP in the trimeric PII protein GlnK2 from Archaeoglobus fulgidus. In: J. Molec. Biol. 402(1), 2010. pp. 165-177.

  • Loqué, D. et al.: Pore Mutations in Ammonium Transporter Amt1 with Increased Electrogenic Ammonium Transport Activity. In: J. Biol. Chem. 284(37), 2009. pp. 24988-24995.

  • Andrade, S.L.A. et al.: Crystal Structure of the NADH:quinone oxidoreductase WrbA from Escherichia coli. In: J. Bacteriol. 189, 2007. pp. 9101-9106.

  • Andrade, S.L.A., Einsle, O.: The Amt/Mep/Rh Family of Ammonium Transport Proteins. In: Molec. Memb. Biol. 24, 2007. pp. 357-365.

  • Einsle, O. et al.: Assignment of Individual Metal Redox States in a Metalloprotein by Crystallographic Refinement at Multiple X-ray Wavelengths. In: J. Am. Chem. Soc. 129, 2007. pp. 2210-2211.

  • Andrade, S.L.A. et al.: Crystal Structure of the Archaeal Ammonium Transporter Amt-1 from Archaeoglobus fulgidus. In: Proc. Natl. Acad. Sci. USA 102, 2005. pp. 14994-14999.

  • Andrade, S.L.A. et al.: Structures of the Iron-Sulfur Flavoproteins from Methanosarcina thermophila and Archaeoglobus fulgidus. In: J. Bacteriology 187, 2005. pp. 3848-3854.

  • Andrade, S.L.A. et al.: Kinetic behavior of Desulfovibrio gigas Aldehyde Oxidoreductase encapsulated in Reverse Micelles. In: Biochem. Biophys. Res. Comm. 308, 2003. pp. 73-78.

  • Einsle, O. et al.: The Nitrogenase MoFe Protein at 1.16 Å Resolution: A Central Ligand in the FeMo Cofactor. In: Science 297, 2002. pp. 1696-1700.

  • Andrade, S.L.A. et al.: Aldehyde oxidoreductase Activity in Desulfovibrio alaskensis NCIMB 13491 – EPR Structural Assignment of the [2Fe-2S] Cluster to the Mo site. In: Eur. J. Biochem 267, 2000. pp. 2054-2061.



Complete list of publications

Selected projects

  • Ammonium transport across prokaryotic and eukaryotic membranes: Structure and function of Amt proteins

Membership in scientific bodies/juries

  • German Research Foundation/Deutsche Forschungsgemeinschaft (DFG)

  • Research Council for Biosciences and Environment (RCBE), Academy of Finland

Additional qualifications

  • MAX INF workshop on experimental phasing of macromolecules, University of Göttingen, Germany, 2006.

  • Course on membrane proteins: the rocky road from gene to structure, Instituto de Tecnologia Química e Biologica (ITQB), Oeiras, Portugal, 2005.

  • "RapiData2005" – Rapid Data Collection and Structure Solving at the NSLS: A practical course in macromolecular X-ray diffraction measurement, Biology Department and National Synchrotron Light Source Brookhaven National Laboratory, New York, USA, 2005.

  • MAX INF workshop on methods in crystallography, University of Göttingen, 2003.

  • Course on molecular structure determination by X-Ray diffraction, Faculty of Sciences and Technology, Universidade Nova de Lisboa, Lisbon, Portugal, 2001.

  • Course on electron paramagnetic resonance – principles and applications to biology, Advanced Studies Centre of Coimbra, Department of Neurosciences, University of Coimbra, Coimbra, Portugal, 2000.

  • EU-ESF advanced practical training course on chemistry of metals in biological systems, Catholic University of Louvain-la-Neuve, Belgium, 1998.

  • Course on X-Ray crystallography and NMR of metalloproteins, Technical Institute of Lisbon, 1998.


Soft Skills/Other activities and achievements

Additional interests

  • Research


Soft Skills

  • 1 daughter, parental leave (50 %), 2009

  • German language course: EUROCENTER – Language Institute, Lisbon, Portugal, 1986 – 1988

  • First Certificate in English: Cambridge Institute, Lisbon 1985


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News

  1. Rhesus Proteins Transport Ions, Not Gas

    Do these proteins carry the gas ammonia or the ammonium ion across cell membranes? Biochemists have long speculated about the details of the ammonium transport proteins (Amt), which include the Rhesus protein factors. Researchers from Freiburg, Germany were able to show experimentally that indeed the ion is carried across the membrane, not the gas.