Carter Dewey S. and Sjögren C.: The SMC complexes, DNA and chromosome topology ? right or knot? Invited review. Crit. Rev. Biochem. Betts Lindroos H., Ström. L., Itoh T., Katou, Y., Shirahige K., and Sjögren C. Chromosomal association of the Smc5/6 complex reveals that it functions in differently regulated pathways. Mol. Cell, 2006 (June 23), 22; 755- 767.
This publication presents the first steps towards an identification of a non-repair function of the Smc5/6 complex.
Ström L., Karlsson C., Betts Lindroos H., Wedahl S., Katou Y., Shirahige K., Sjögren C. Post-replicative formation of cohesion is required for repair and induced by a single DNA break. Science, 2007; 317; 242-245.
This article presents results showing that DNA damage induces a signalling cascade which trigger de novo formation of sister chromatid cohesion in post-replicative cells. This contradicted the then current idea that genome-wide cohesion only was created in connection to the replication fork.
Kegel A. and Sjögren C. The Smc5/6 complex – more than repair? Cold Spring Harb Symp Quant Biol. 2010;75:179-87.
In this review we present and discuss the accumulating evidence for a non-repair function of Smc5/6 complex. This function has since 2007 been one of the main objects of investigations in my research team.
Kegel A., Betts-Lindroos H., Kanno K., Jeppsson K., Ström L.,Katou Y., Itoh T., Shirahige S., Sjögren C. Chromosome length influences replication-induced topological stress. Nature, 2011; 471; 392-396.
This article presents our continued analysis of the Smc5/6 complex which indicated that replication-induced supercoiling is diffusible over chromosome ends. This was contrary to then current idea that supercoiling was confined to smaller chromosomal domains.
Carter Dewey S. and Sjögren C. The SMC complexes, DNA and chromosome topology –right or knot? Invited review. Crit. Rev. Biochem. Mol. Biol. 2012; 47;1-16
Here we present and discuss observations which indicate that SMC complexes control, and are controlled by, DNA supercoiling.
Lilienthal I, Kanno T, Sjögren C. Inhibition of the Smc5/6 complex during meiosis perturbs joint molecule formation and resolution without significantly changing crossover or non-crossover levels. PLoS Genet., 2013; 9(11): e1003898. doi: 10.1371/journal.pgen.1003898.
This article deals with the repair function of Smc5/6 and present results showing that the Smc5/6 complex controls the formation and/or resolution of recombination intermediates between sister chromatids during the meiotic cell cycle.
Jeppsson K., Carlborg K., Nakato R., Berta D., Lilienthal I., Kanno T., Lindqvist A., Brink M., Dantuma N., Katou Y., Shirahige K., Sjögren C. The Chromosomal Association of the Smc5/6 Complex Depends on Cohesion and Predicts the Level of Sister Chromatid Entanglement. PLoS Genet., 2014; doi: 10.1371/journal.pgen.1004680.
In this article we show that the Smc5/6 complex preferentially associates to entangled sister chromatids and provide evidence that the level of entanglement is under control of replication-induced supercoiling.
Jeppsson K., Kanno T., Shirahige K., and Sjögren C. The maintenance of chromosome structure: positioning and function of SMC complexes. Invited review, Nat. Rev. Mol. Cell Biol. 2014; 15; 601-14
In this overview of the field we present, compares and discusses the then current knowledge of the chromosomal association of different SMC complexes. The invitation to write this review indicates that the work from my laboratory is appreciated by the scientific community.
Kanno T., Berta D., Sjögren C. The Smc5/6 complex is an ATP-dependent intermolecular DNA linker. Cell reports, 2015, doi: 10.1016/j.celrep.2015.07.048.
The presented biochemical and in vivo analysis provides the first detailed analysis of how the Smc5/6 complex binds DNA and chromosomes. It also and unravels a physical interaction between the complex and topoisomerase 2, thereby providing a direct connection between Smc5/6 complex and the regulation of DNA supercoiling and entanglement (by topoisomerase 2).